World Journal of Peri and Neonatology <p><strong>World Journal of Peri &amp; Neonatology (WJPN)</strong> is a peer-reviewed and free-access journal founded in 2017, by Mother and Newborn Health Care Center, Yazd, Iran. The Journal publishes articles in English across on all aspects of preconceptional, perinatal, and postpartum periods; and of fetuses and newborn infants during and beyond their initial hospital stay. The Editorial Board is comprised of an Editor-in-Chief and Associate Editors, highly qualified scientists of different institutions across Iran or overseas.The WJPN audience includes specialist in obstetrics, neonatology, perinatology, maternal/fetal medicine, and neonatal and perinatal nurses. It publishes peer-reviewed clinical research articles, review or update articles, case reports, commentaries,editorials and letters to the Editor. However, other types of articles may be included depending on the interest of the Journal or the need to&nbsp;publish relevant subject matters not suitable for the categories above.</p> en-US (Dr Mahta Mazaheri MD, PhD) (Arman Research Institute) Sat, 18 Nov 2017 00:00:00 +0330 OJS 60 Short-term High Dose of Quercetin and Resveratrol Alters Aging Markers in Human Kidney Cells <p>Abstract<br>Background: Hyperglycemia-mediated oxidative stress implicates in etiology of kidney cell aging and<br>diabetic nephropathy. We evaluated the effects of different doses of resveratrol and quercetin and their<br>combination therapy on aging marker in human kidney cell culture under hyperglycemia condition.<br>Methods: Human embryonic kidney cell (HEK-293) was cultured in Dulbecco’s Modified Eagle<br>Medium (DMEM) containing 100 mM (18 mg/L) for 24 h. The cells were treated with resveratrol<br>(2.5, 5, 10 μm), quercetin (3, 6, 12 μm), and combination of these (R 2.5 μm, Q 3 μm) and (R 5 μm,<br>Q 6 μm) and (R 10 μm, Q 12 μm) for 48 h, and then, cells were lysed to access RNA and lysate.<br>Results: The analysis of data showed that beta-galactosidase enzyme gene expression as an aging<br>marker in all treatment groups has reduced in a dose-dependent manner. Gene expression of Sirtuin1<br>and thioredoxin (Trx) in all treated groups in comparison to control group increased in a dose-dependent<br>fashion. Trx interacting protein (TXNIP) gene expression decreased in a dose-dependent manner in all<br>treated groups, especially in resveratrol and combination therapy. Conclusions: According to the results<br>of this research, quercetin, resveratrol, and especially combination treatments with increased expression<br>levels of antioxidants, can reduce aging markers in HEK cell line in hyperglycemia conditions. These<br>results lead us to use flavonoids such as resveratrol for anti-aging potential.<br>Keywords: Aging, human embryonic kidney cell-293, quercetin, resveratrol, thioredoxins</p> amir bozorgzad ##submission.copyrightStatement## Sat, 18 Nov 2017 00:00:00 +0330 Comparison of the prevalence of enteroviruses in blood samples of patients with and without unstable angina <p>BACKGROUND: Although the role of enteroviruses has been proved in heart diseases, extensive information is not available on the association between enteroviruses and unstable angina. In the present study, the authors compared the prevalence of enteroviruses in patients with and without unstable angina. METHODS: Blood samples were taken from 51 patients with unstable angina and 55 patients without unstable angina or myocardial infarction that were admitted to Imam Reza and Ghaem hospitals (Mashhad, northeast of Iran). Reverse transcription polymerase chain reaction (RT-PCR) was performed using specific primers for the detection of the enteroviruses in blood samples of study subjects. RESULTS: Patients with and without unstable angina were similar in age with mean ± standard deviation of 62.6 ± 12.8 and 59.7 ± 12.7 years, respectively (P = 0.243) and there were no differences in gender in these two groups (P = 0.174). Prevalence of the enteroviruses in patients with unstable angina was higher only in 66-80 years age group compared to the control group (patients without unstable angina, P = 0.032). There was a higher prevalence of enterovirus RNA positivity in the blood samples of women with unstable angina (75.9%) than those without unstable angina (41.7%, P = 0.011), however, no significant difference was observed in men (P = 0.983). CONCLUSION: Our data showed that enteroviral RNA positivity was higher in patients with unstable angina compared to those without unstable angina. However, the differences between the two groups were not statistically significant. Keywords: Unstable Angina, Enterovirus, Reverse Transcriptase PCR</p> hamideh khorram ##submission.copyrightStatement## Sat, 18 Nov 2017 00:00:00 +0330