World Journal of Peri and Neonatology <p><strong>World Journal of Peri &amp; Neonatology (WJPN)</strong> is a peer-reviewed and free-access journal founded in 2017, by Mother and Newborn Health Care Center, Yazd, Iran. The Journal publishes articles in English across on all aspects of preconceptional, perinatal, and postpartum periods; and of fetuses and newborn infants during and beyond their initial hospital stay. The Editorial Board is comprised of an Editor-in-Chief and Associate Editors, highly qualified scientists of different institutions across Iran or overseas.The WJPN audience includes specialist in obstetrics, neonatology, perinatology, maternal/fetal medicine, and neonatal and perinatal nurses. It publishes peer-reviewed clinical research articles, review or update articles, case reports, commentaries,editorials and letters to the Editor. However, other types of articles may be included depending on the interest of the Journal or the need to&nbsp;publish relevant subject matters not suitable for the categories above.</p> SamandPub en-US World Journal of Peri and Neonatology Association of MTHFR A1298C Polymorphism with Preterm Birth: A Meta-Analysis <p><strong>Background:</strong> A few studies have been conducted to explore the association of MTHFR A1298C (rs1801131) polymorphism with preterm birth risk, the results remain inconsistent. Therefore, we conducted a meta-analysis to derive a more systematic estimation of the association.</p> <p><strong>Method:</strong> Relevant studies were searched by PubMed, EMBASE, CNKI, and Google Scholar up to June 2018. The strength of the association of MTHFR A1298C polymorphism with preterm birth was calculated by odds ratios (OR) with 95% confidence interval (95%CI).</p> <p><strong>Results:</strong> A total of nine case-control studies with 1,609 cases and 14,981 controls were included. Pooled results showed that there was no significant association between MTHFR A1298C polymorphism and preterm birth risk under all five genetic models in overall. However, in the stratified analysis of ethnicity, a significant association between MTHFR A1298C polymorphism and preterm birth risk was observed in the Asians under four genetic models, i.e., allele (C vs. A: OR = 0.960, 95% CI 0.543-0.871, P = 0.002), heterozygote (CA vs. AA: OR = 0.887, 95% CI 0.024-0.457, P = 0.003), dominant (CC+CA vs. AA: OR = 0.965, 95% CI 0.534 -0.935, P = 0.015) and recessive (CC vs. CA+AA: OR = 0.923, 95% CI 0.026-0491, P = 0.004), but not in Caucasians.</p> <p><strong>Conclusion:</strong> This meta-analysis suggested that MTHFR A1298C polymorphism is not associated with preterm birth risk in overall population. However, MTHFR A1298C polymorphism plays an important role in preterm birth development in Asian population.</p> <p><strong>Keywords:</strong> Preterm Birth, MTHFR Gene, Polymorphism, Meta-analysis</p> Atiyeh Javaheri Sahel Khajehnoori Elnaz Foroughi Rezvan Nasiri Soudabeh Farahnak Razieh Sadat Tabatabaei Hossein Neamatzadeh ##submission.copyrightStatement## 2018-07-02 2018-07-02 1 6 Prevalence of Congenital Anomalies in Newborns in Zabol: A Single Hospital Based Study <p><strong>Background:</strong> Congenital anomalies are conditions of prenatal origin that are present at birth, potentially impacting an infant's health, development and survival. The birth of a newborn with congenital anomalies imposes financial and emotional burdens on families. The aim of this study was to investigate the epidemiology of congenital anomalies in 2014 in Zabol, Iran.</p> <p><strong>Methods:</strong> This cross-sectional study was carried out in the Amir Al-Momenin Hospital between May of 2013 and April 2014. All the live born were included, and we have examined all newborns for the presence of congenital anomalies.</p> <p><strong>Results:</strong> During the twelve months, 9,309 newborns were included to the study. Of them, 72 (0.8%) newborns were affected with a congenital malformation. The congenital anomalies affected significantly higher proportion of male newborns (66.7%) than female newborns (33.3%). Among of them, musculoskeletal system abnormalities account for 45.9% of the total abnormalities allocating the highest frequency to itself. Subsequently, central nervous system abnormality (19.4%), gastrointestinal anomaly (15.3%), urinary tract abnormality (9.7%), chromosomal abnormality (2.8%), and multiple anomalies (1.4%) were frequent, respectively.</p> <p><strong>Conclusion:</strong> This study showed that prevalence of congenital anomalies is restively high in Zabol city. The results of this study identify the necessity of genetic counseling and early diagnosis to prevention, care and surveillance.</p> <p><strong>Keywords:</strong> Congenital anomaly, Chromosomal abnormality, Prenatal, Counseling</p> Mojtaba Jahantiq Mahta Mazaheri Tayebeh Rabaninia Atiyeh Javaheri Nooshin Amjadi ##submission.copyrightStatement## 2018-07-02 2018-07-02 7 14 A Survey on the Relationship between Ages above 35 Years and Gestational Diabetes Mellitus <p><strong>Background:</strong> The aim of this study was to determine the relationship between ages above 35 years old and gestational diabetes mellitus in Zabol.</p> <p><strong>Methods:</strong> This study was conducted in Zabol city during 2016-2017. Analyzing the data to check the validity of the hypothesis in any type of research is of particular importance and is performed nowadays in most research studies based on the data gathered from the subjects under study. Data analysis is one of the main and most important parts of any research. Raw data is analyzed using statistical software.</p> <p><strong>Results: </strong>The results of this study showed that according to the gestational diabetes mellitus score of 150 mothers, about gestational diabetes, 11 cases (7.3%) had gestational diabetes, and 132 (92.7%) others have not experienced gestational diabetes. Generally, according to the average score (1.92), there is no gestational diabetes in mothers.</p> <p><strong>Conclusion:</strong> The most important risk factors for gestational diabetes are age-obesity and familial history, respectively.</p> <p><strong>Keywords:</strong> Gestational diabetes, High-age, Zabol city</p> Kolsoum Rezaie Kahkhaie Ashrafali Rezaie Kehkhaie Leili Rezaie Kahkhaie Maryam Koochakzai Khadijeh Rezaie Keikhaie Maryam Nakhaee Moghaddam ##submission.copyrightStatement## 2018-07-02 2018-07-02 15 20 Isolation of Beta-Lactamase Producing Genes (SHV, CTX-M1, CTX-M2, CTX-M3) in Escherichia coli Isolated from Pregnant Woman Patients <p><strong>Background:</strong> In recent decades, extended spectrum beta-lactamase (ESBL) generating bacteria have increased universally. Among the most important causative agents of nosocomial infections throughout the world, <em>Escherichia coli </em>as main ESBL-producing bacteria are so highly regarded. Trends in the treatment of infections by such bacteria have led to a global concern.</p> <p><strong>Methods:</strong> All strains were cultured and identified by the Clinical Microbiology Laboratory and were recovered from blood and urine cultures. <em>In-vitro</em> presence of ESBL was confirmed with Clinical and Laboratory Standard Institute double disc and PCR for CTX-M1, CTM-M2, CTX-M3 method.</p> <p><strong>Results: </strong>The results of this study showed that Escherichia coli samples were resistant to AN (42.85%), GM (28.57%), AM (35.71%), AMC (35.71%), CZ (35.71%), and AZM (50%) antibiotics. While the most susceptible to antibiotic was ampicillin (64.28%), the least resistance to antibiotics was gentamicin.</p> <p><strong>Conclusion:</strong> The current situation of multiple bacterial antibiotic resistance has become a worrisome issue in UTI. Multi-drug-resistant <em>E. coli</em> can be readily encountered in hospital settings during daily clinical practice, and urologist should act timely. The management of such infections is extremely important for the future, with particular reference to prevention of new antibiotic resistance patterns.</p> <p><strong>Keywords:</strong> <em>E.coli</em>, Extended-spectrum beta-lactamase, PCR, Gene CTX, SHV</p> Kolsoum Rezaie Kahkhaie Ashrafali Rezaie Kehkhaie Leili Rezaie Kahkhaie Maryam Koochakzai Khadijeh Rezaie Keikhaie Maryam Nakhaee Moghaddam ##submission.copyrightStatement## 2018-07-02 2018-07-02 21 29 Association between IRF6 rs642961 Polymorphism and Nonsyndromic Cleft Lip with or without Cleft Palate Risk in an Iranian Population <p><strong>Background:</strong> The extent of the contribution of rs642961 polymorphism of interferon regulatory factor-6 (IRF6) gene with susceptibility to the syndromic cleft lip with or without cleft palate (NSCL/P) in the Iranian patients is still unknown. Thus, to test the role of IRF6 in NSCL/P susceptibility in an Iranian population, we performed a population based case-control stud.</p> <p><strong>Methods:</strong> One-hundred ten patients with NSCL/P and 110 matched healthy subjects were recruited to this population-based study. The IRF6&nbsp;rs642961 polymorphism was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay.</p> <p><strong>Results: </strong>Participants did not differ significantly by age and gender <br> (P &gt; 0.05). The AA, AG, and GG genotypes frequencies of the IRF6&nbsp;rs642961 polymorphism in the NSCL/P cases were 27.3%, 53.6% and 19.1%, respectively while the corresponding frequencies in the healthy subjects were 34.5, 56.4% and 9.1%, respectively. There was a significant association between homozygote mutant genotype (GG) of IRF6&nbsp;rs642961 polymorphism and increased risk of NSCL/P (OR = 2.360, 95% CI 1.055-5.280, P = 0.037).</p> <p><strong>Conclusion:</strong> the current study suggested that IRF6&nbsp;rs642961 polymorphism might be associated with susceptibility to NSCL/P in an Iranian population. However, well-designed epidemiological studies with larger sample size are needed to further validate our results.</p> <p><strong>Keywords:</strong> Nonsyndromic, Cleft lip, Cleft palate, Interferon regulatory factor 6, Polymorphism</p> Seyed Mohammadreza Niktabar Abdolhamid Amooee Parisa Piroozmand Soudabeh Farahnak Elnaz Foroughi Rezvan Nasiri Seyed Mojtaba Latifi Hossein Neamatzadeh ##submission.copyrightStatement## 2018-07-03 2018-07-03 30 35 Genetic Disorders in the Fetus from Diagnosis to Treatment <p><strong>Background:</strong> Today there are many diseases that genetics contributes to these, including diabetes, hypertension, cancer, heart disease, various types of cancers and even mental illness, and by the advancement of medical knowledge The number of genetic diseases is increasing now. most people think about genetic diseases as inherited diseases, which are mostly caused by gene mutations and other genetically inherited factors. Most of the genetic disorders currently do not have 100% treatment, and the only way is to preventing the prevalence of them before marriage.</p> <p><strong>Methods:</strong> Eligible articles were identified by search of databases including NCBI, PubMed, ISI Web of Knowledge and Google scholar up to July 2017.</p> <p><strong>Results:</strong> In the field of prevention of disability and genetic disorders, many members who are at risk of genetic disease should have genetic counseling to get useful information about it. Without genetic counseling, genetic testing is meaningless, as people do not go to the laboratory without a doctor's visit; Genetic counselors won’t be able to stop people from getting married, but they provide solutions to prevent them from possible disorders.</p> <p><strong>Conclusions:</strong> This study provides information on genetic diseases and prevents them in newborns and suggests new solutions for the treatment of these diseases.</p> <p><strong>Keywords: </strong>Gnetic, Pre-implantation Dagnosis, Genetic counseling, Congenital</p> Tayebeh Rabaninia Nooshin Amjadi ##submission.copyrightStatement## 2018-07-03 2018-07-03 36 47 CRISPR Gene Editing on Human Embryos <p><strong>Background: </strong>With the recent development of CRISPR/Cas9 genome editing technology, the possibility to genetically influence the human germline (gametes and embryos) has become a separate technical possibility. As a powerful skill for genome engineering, the CRISPR/Cas9 system has been effectively applied to adjust the genomes of several species. The purpose of this review was to appraise the technology and build concepts for the launch of precise hereditary modifications in early human embryos.</p> <p><strong>Methods:</strong> We conducted a systematic review of the related literatures searched from PubMed, Google scholar, Web of Science up to June 30, 2017 and then we extracted the essential data. In this review, we present the brief history and basic mechanisms of the CRISPR/Cas9 system and significant challenges and advances in the field as a comprehensive practical guide to absorbed users of genome editing technologies. We introduce factors that influence CRISPR/Cas9 efficacy which must be addressed before effective&nbsp;in vivo&nbsp;human embryo therapy can be realized .in this review, we highlight the advancements that have been made using CRISPR/Cas9 in relation to Human Embryo.</p> <p><strong>Results and Conclusion: </strong>The possibility of CRISPR/Cas9 use in the context of human reproduction, to change embryos, germline cells, and pluripotent stem cells are studied created on the writers' expert belief. We discuss recent developments leading to the operation of Human Embryonic gene therapies in clinical trials and consider the predictions for future advances in this rapidly developing field.<strong><br></strong></p> <p><strong>Keywords: </strong>Gene editing, Gene therapy, Development, Embryo, Reproduction</p> Fatemeh Sefid Saedeh Khadempar Roshanak Shamriz Nooshin Amjadi ##submission.copyrightStatement## 2018-07-03 2018-07-03 48 55 A Girl with Farber Disease Treated with Bone Marrow Transplantation <p><strong>Background: </strong>Farber disease is a very rare autosomal recessive disease of lipid metabolism caused by deficient activity of lysosomal acid ceramidase. Symptoms can begin in the first year of life by a triad of painful and swollen joints and subcutaneous nodules, progressive hoarseness and variable central nervous system involvement.</p> <p><strong>Case Report: </strong>A 5 months old girl with subcutaneous nodules in limbs, pain and swelling in her fingers, Knees, elbow and hoarseness was referred to our clinic. She had neurodevelopment delay in walking and talking. Genetic analysis was reported homozygosity for a c.830C&gt;A mutation in exon 11 of N-Acylsphingosine Amidohydrolase 1 (ASAH1) gene. She diagnosed with Farber disease and treated with bone marrow transplantation. After that her signs and symptoms were improved and she could to walk.</p> <p><strong>Conclusion: </strong>Farber disease is associated with characteristics including swollen joints, subcutaneous nodules, progressive hoarseness and variable CNS involvement. Moreover, bone marrow transplantation improved these symptoms.</p> <p><strong>Keywords:</strong> Farber disease, Bone marrow transplantation, CNS involvement</p> Naser Ali Mirhosseini Elham Farasat Elnaz Sheikhpour ##submission.copyrightStatement## 2018-07-03 2018-07-03 56 58