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Background: Hyperglycemia-mediated oxidative stress implicates in etiology of kidney cell aging and
diabetic nephropathy. We evaluated the effects of different doses of resveratrol and quercetin and their
combination therapy on aging marker in human kidney cell culture under hyperglycemia condition.
Methods: Human embryonic kidney cell (HEK-293) was cultured in Dulbecco’s Modified Eagle
Medium (DMEM) containing 100 mM (18 mg/L) for 24 h. The cells were treated with resveratrol
(2.5, 5, 10 μm), quercetin (3, 6, 12 μm), and combination of these (R 2.5 μm, Q 3 μm) and (R 5 μm,
Q 6 μm) and (R 10 μm, Q 12 μm) for 48 h, and then, cells were lysed to access RNA and lysate.
Results: The analysis of data showed that beta-galactosidase enzyme gene expression as an aging
marker in all treatment groups has reduced in a dose-dependent manner. Gene expression of Sirtuin1
and thioredoxin (Trx) in all treated groups in comparison to control group increased in a dose-dependent
fashion. Trx interacting protein (TXNIP) gene expression decreased in a dose-dependent manner in all
treated groups, especially in resveratrol and combination therapy. Conclusions: According to the results
of this research, quercetin, resveratrol, and especially combination treatments with increased expression
levels of antioxidants, can reduce aging markers in HEK cell line in hyperglycemia conditions. These
results lead us to use flavonoids such as resveratrol for anti-aging potential.
Keywords: Aging, human embryonic kidney cell-293, quercetin, resveratrol, thioredoxins